小尾寒羊间情期和发情期microRNAs差异表达分析

doi:10.11843/j.issn.0366-6964.2016.07.004

Abstract

Abstract:

To explore the theoretical basis of the microRNAs in regulating the reproductive process in Small Tail Han sheep，the ovarian microRNAs profiles were constructed and analyzed in estrus and diestrus.One ovary was collected with surgery method at estrus (the 1st day) and diestrus (the 13th day) from the same sheep，respectively.The RNA sequencing data were obtained with illumina Hiseq2000 sequencing platform.The expression profiles and differential expression of microRNAs were analyzed by bioinformatics method.The real-time quantitative PCR (qRT-PCR) was used for validating the differentially expressed microRNAs between the 2 periods in ovary.The microRNAs expression profiles of Small Tailed Han sheep was constructed in the period of estrus and diestrus in ovaries.The highest expression of microRNA was observed for oar-miR-99a and oar-miR-143 in the period of diestrus and estrus，respectively.Three microRNAs，oar-miR-200a，oar-miR-200b and oar-miR-200c，were significantly differentially expressed between estrus and diestrus.The expression level of 2 randomly selected microRNAs by qRT-PCR was the same as the result by RNA-seq data.These microRNAs expression profiles will be used to provide informations for further studying the microRNAs in sheep ovary.The differentially expressed microRNAs may regulate the ovarian periodic activity through the metabolism and immunity pathway by the analysis of predicted target genes and enriched pathways.

CLC Number:

S826
S813.3

DUAN Xin-chong，WEI Yan-hui，LI Yang，LI Xiang-yun，ZHOU Rong-yan，XI Jian-zhong. Analyzing the Differential Expression of microRNAs in Estrus and Diestrus of Small Tailed Han Sheep[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2016, 47(7): 1324-1332.

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Analyzing the Differential Expression of microRNAs in Estrus and Diestrus of Small Tailed Han Sheep

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